Mechanics of induced pluripotent stem cell cysts

Encapsulation of human induced pluripotent stem cells (hIPSC) inside alginate capsules qithe the appropriate niche-like environment allows the formation of lumenized three-dimensional colonies that significantly improve viabilityand expansion rates as compared with standard hPSC. We are now investigating in details tha growth, organization and mechanical properties of these stem cell cysts.

• Cohen, P. J., Luquet, E., Pletenka, J., Leonard, A., Warter, E., Gurchenkov, B., …Nassoy, P., … & Alessandri, K. (2023). Engineering 3D micro-compartments for highly efficient and scale-independent expansion of human pluripotent stem cells in bioreactors. Biomaterials, 122033.